Construction of miRNA-mRNA Regulatory Network and Prognostic Signature in Endometrial Cancer.

ABSTRACT:

Introduction

This bioinformatic study confirmed a new miRNA-mRNA regulatory network and a prognostic signature in endometrial cancer (EC).

Materials and methods

We downloaded RNA-seq and miRNA-seq data of EC from the TCGA database, then used EdegR package to screen differentially expressed miRNAs and mRNAs (DE-miRNAs and DE-mRNAs). Then, we constructed a regulatory network of EC-associated miRNAs and hub genes by Cytoscape, and determined the expression of unexplored miRNAs in EC tissues and normal adjacent tissues by quantitative Real-Time PCR (qRT-PCR). A prognostic signature model and a predictive nomogram were constructed. Finally, we explored the association between the prognostic model and the immune cell infiltration.

Results

A total of 11,531 DE-mRNAs and 236

SUBMITTER: Sun R 

PROVIDER: S-EPMC8039850 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature