Dataset Information

Basilaphelenchus hyrcanus n. sp. (Rhabditida: Tylaphelenchinae) associated with bark of a beech tree (Fagus orientalis Lipsky) from northern Iran.

ABSTRACT: Basilaphelenchus hyrcanus n. sp., the sixth species of the newly established genus was isolated during a nematode survey associated with bark samples of beech tree in northern Iran, which differs from the other species by body size, stylet length, metacorpus structure, and tail characters of both genders. The new species is also characterized by elevated cephalic region with sclerotised vestibule, posteriorly directed stylet knobs, well-developed metacorpus filling corresponding body region, position of excretory pore at the level of base of metacarpus, length of post uterine sac by 38-60 μm long, conoid elongate tail by sharp to finely rounded tip in female. Based upon the morphological characteristics and phylogenetic analyses of partial 18 S and D2-D3 28 S rDNA segments, the new species mostly resembles B. magnabulbus. However, B. hyrcanus n. sp. is clearly different from this species by having a longer stylet, different position of the excretory pore, a different male tail tip characters and 4.4 and 13.2% sequence divergences in 18 S and D2-D3 28 S, respectively.

SUBMITTER: Golhasan B

PROVIDER: S-EPMC8039998 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Basilaphelenchus hyrcanus n. sp. (Rhabditida: Tylaphelenchinae) associated with bark of a beech tree (Fagus orientalis Lipsky) from northern Iran.

Golhasan Behrouz B Miraeiz Esmaeil E Tanha Maafi Zahra Z Heydari Ramin R

Journal of nematology 20210317

Basilaphelenchus hyrcanus n. sp., the sixth species of the newly established genus was isolated during a nematode survey associated with bark samples of beech tree in northern Iran, which differs from the other species by body size, stylet length, metacorpus structure, and tail characters of both genders. The new species is also characterized by elevated cephalic region with sclerotised vestibule, posteriorly directed stylet knobs, well-developed metacorpus filling corresponding body regi ...[more]

PMID: 33860258

Similar Datasets

Project description:The Hyrcanian forest in northern Iran is threatened by human use and encroachment and has suffered degradation in some areas. The forest has been declared a World Heritage Site and management in the region is shifting from timber production to conservation. There is considerable interest in developing a greater understanding of these diverse forest communities to inform forest management and multiple use plans to maintain the diversity and resilience of these forests. The Hyrcanian forest is characterized by a complex topography of catenas ranging up mountain slopes. Topographic gradients greatly influence microhabitat conditions which in turn impact tree distribution. To date there has been limited research on the impacts of this diverse topography on the spatial distribution of tree species and tree diameters in Hyrcanian forests. Such information is necessary to better understand the regional traits of tree diameters in these natural mixed temperate forests before forest management occurs. We examined the influence of the area's catena topography on the spatial pattern of tree species and on species stand structure in terms of tree diameter distribution. To quantify these dynamics, we conducted a complete enumeration inventory of all trees with dbh >12 cm within a 7.947 ha study area that included three C-shaped (concave) and three V-shaped (convex) catenas. Geostatistical variogram analysis and Clark and Evans aggregation index were utilized to study the spatial distribution of tree diameters. Beech, alder, hornbeam, linden and Persian maple exhibited clustered patterns, and sour cherry, ash, and oak exhibited random patterns. Geostatistical analysis clearly revealed the substantial influence of catena topography on the diameter distributions of alder and linden, more subtle influence on the diameter distributions of beech, and a possible influence on Persian maple, providing valuable insight into stand structure over neighborhood-based indices alone. Alder and linden both exhibited strong spatial structure in their diameter distributions (56% and 86%, respectively) where their diameter was strongly correlated with trees within 108 m and 83 m, respectively, sharing more similar diameters to each other than trees beyond that distance. Beech, maple, and hornbeam exhibited very weak if any spatial structure over short distances. These findings can be used to support the alignment of forest management practices in managed Hyrcanian forests with goals of protecting and maintaining biodiversity and sustainable forest ecosystems, and to inform geospatial modeling of species diameter distributions in areas where a complete stem-map is not feasible.

Project description:UnlabelledBackgroundBeech bark disease is an insect-fungus complex that damages and often kills American beech trees and has major ecological and economic impacts on forests of the northeastern United States and southeastern Canadian forests. The disease begins when exotic beech scale insects feed on the bark of trees, and is followed by infection of damaged bark tissues by one of the Neonectria species of fungi. Proteomic analysis was conducted of beech bark proteins from diseased trees and healthy trees in areas heavily infested with beech bark disease. All of the diseased trees had signs of Neonectria infection such as cankers or fruiting bodies. In previous tests reported elsewhere, all of the diseased trees were demonstrated to be susceptible to the scale insect and all of the healthy trees were demonstrated to be resistant to the scale insect. Sixteen trees were sampled from eight geographically isolated stands, the sample consisting of 10 healthy (scale-resistant) and 6 diseased/infested (scale-susceptible) trees.ResultsProteins were extracted from each tree and analysed in triplicate by isoelectric focusing followed by denaturing gel electrophoresis. Gels were stained and protein spots identified and intensity quantified, then a statistical model was fit to identify significant differences between trees. A subset of BBD differential proteins were analysed by mass spectrometry and matched to known protein sequences for identification. Identified proteins had homology to stress, insect, and pathogen related proteins in other plant systems. Protein spots significantly different in diseased and healthy trees having no stand or disease-by-stand interaction effects were identified.ConclusionsFurther study of these proteins should help to understand processes critical to resistance to beech bark disease and to develop biomarkers for use in tree breeding programs and for the selection of resistant trees prior to or in early stages of BBD development in stands. Early identification of resistant trees (prior to the full disease development in an area) will allow forest management through the removal of susceptible trees and their root-sprouts prior to the onset of disease, allowing management and mitigation of costs, economic impact, and impacts on ecological systems and services.

Dataset Information

Basilaphelenchus hyrcanus n. sp. (Rhabditida: Tylaphelenchinae) associated with bark of a beech tree (Fagus orientalis Lipsky) from northern Iran.

Publications

<i>Basilaphelenchus hyrcanus</i> n. sp. (Rhabditida: Tylaphelenchinae) associated with bark of a beech tree (<i>Fagus orientalis</i> Lipsky) from northern Iran.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets