Loss of hepatic Mboat7 leads to liver fibrosis.

ABSTRACT:

Objective

The rs641738C>T variant located near the membrane-bound O-acyltransferase domain containing 7 (MBOAT7) locus is associated with fibrosis in liver diseases, including non-alcoholic fatty liver disease (NAFLD), alcohol-related liver disease, hepatitis B and C. We aim to understand the mechanism by which the rs641738C>T variant contributes to pathogenesis of NAFLD.

Design

Mice with hepatocyte-specific deletion of MBOAT7 (Mboat7^Δhep) were generated and livers were characterised by histology, flow cytometry, qPCR, RNA sequencing and lipidomics. We analysed the association of rs641738C>T genotype with liver inflammation and fibrosis in 846 NAFLD patients and obtained genotype-specific liver lipidomes from 280 human biopsies.

Results

Allelic imbalance an

SUBMITTER: Thangapandi VR 

PROVIDER: S-EPMC8040158 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature