Peripheral CD4 T follicular cells induced by a conjugated pneumococcal vaccine correlate with enhanced opsonophagocytic antibody responses in younger individuals.

ABSTRACT:

Background

PCV13 (conjugated polysaccharide) and PPSV23 (polysaccharide only) are two licensed vaccines targeting S. pneumoniae. The role of CD4 T-cell responses in pneumococcal vaccines among healthy participants and their impact on antibodies is not yet known.

Methods

Ten adults (5 old and 5 young) received PCV13 (prime) and a year later PPSV23 (boost). Blood samples were collected prior to and multiple time points after vaccination. CD4 T cells responding to CRM₁₉₇, polysaccharide (PS), CRM₁₉₇ conjugated polysaccharide (CPS), PCV13 and PPSV23 vaccines were measured by flow cytometry. Serum antibodies were analyzed via multiplex opsonophagocytosis (MOPA) and pneumococcal IgG assays.

Results

Vaccine-specific CD4 T cells were induced in all ten v

SUBMITTER: Sterrett S 

PROVIDER: S-EPMC8040292 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature