Integrin Activation Enables Sensitive Detection of Functional CD4⁺ and CD8⁺ T Cells: Application to Characterize SARS-CoV-2 Immunity.

ABSTRACT: We have previously shown that conformational change in the β₂-integrin is a very early activation marker that can be detected with fluorescent multimers of its ligand intercellular adhesion molecule (ICAM)-1 for rapid assessment of antigen-specific CD8⁺ T cells. In this study, we describe a modified protocol of this assay for sensitive detection of functional antigen-specific CD4⁺ T cells using a monoclonal antibody (clone m24 Ab) specific for the open, high-affinity conformation of the β₂-integrin. The kinetics of β₂-integrin activation was different on CD4⁺ and CD8⁺ T cells (several hours vs. few minutes, respectively); however, m24 Ab readily stained both cell types 4-6 h after antigen stimulation. With this pr

SUBMITTER: Schollhorn A 

PROVIDER: S-EPMC8040333 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature