Upgraded CRISPR/Cas9 tools for tissue-specific mutagenesis in <i>Drosophila</i>.

ABSTRACT: CRISPR/Cas9 has emerged as a powerful technology for tissue-specific mutagenesis. However, tissue-specific CRISPR/Cas9 tools currently available in Drosophila remain deficient in three significant ways. First, many existing gRNAs are inefficient, such that further improvements of gRNA expression constructs are needed for more efficient and predictable mutagenesis in both somatic and germline tissues. Second, it has been difficult to label mutant cells in target tissues with current methods. Lastly, application of tissue-specific mutagenesis at present often relies on Gal4-driven Cas9, which hampers the flexibility and effectiveness of the system. Here, we tackle these deficiencies by building upon our previous CRISPR-mediated tissue-restricted mutagenesis (CRISPR-TRiM) tools. First,

SUBMITTER: Koreman GT 

PROVIDER: S-EPMC8040800 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature