An extracellular vesicle-related gene expression signature identifies high-risk patients in medulloblastoma.

ABSTRACT:

Background

Medulloblastoma (MB) is a malignant brain tumor in childhood. It comprises 4 subgroups with different clinical behaviors. The aim of this study was to characterize the transcriptomic landscape of MB, both at the level of individual tumors as well as in large patient cohorts.

Methods

We used a combination of single-cell transcriptomics, cell culture models and biophysical methods such as nanoparticle tracking analysis and electron microscopy to investigate intercellular communication in the MB tumor niche.

Results

Tumor cells of the sonic hedgehog (SHH)-MB subgroup show a differentiation blockade. These cells undergo extensive metabolic reprogramming. The gene expression profiles of individual tumor cells show a partial convergence with those of tumor-associ

SUBMITTER: Albert TK 

PROVIDER: S-EPMC8041350 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature