Infection with a newly designed dual fluorescent reporter HIV-1 effectively identifies latently infected CD4⁺ T cells.

ABSTRACT: The major barrier to curing HIV-1 infection is a small pool of latently infected cells that harbor replication-competent viruses, which are widely considered the origin of viral rebound when antiretroviral therapy (ART) is interrupted. The difficulty in distinguishing latently infected cells from the vast majority of uninfected cells has represented a significant bottleneck precluding comprehensive understandings of HIV-1 latency. Here we reported and validated a newly designed dual fluorescent reporter virus, DFV-B, infection with which primary CD4⁺ T cells can directly label latently infected cells and generate a latency model that was highly physiological relevant. Applying DFV-B infection in Jurkat T cells, we generated a stable cell line model of HIV-1 latency with diverse

SUBMITTER: Cai J 

PROVIDER: S-EPMC8041464 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature