Developing a non-destructive metabarcoding protocol for detection of pest insects in bulk trap catches.

ABSTRACT: Metabarcoding has the potential to revolutionise insect surveillance by providing high-throughput and cost-effective species identification of all specimens within mixed trap catches. Nevertheless, incorporation of metabarcoding into insect diagnostic laboratories will first require the development and evaluation of protocols that adhere to the specialised regulatory requirements of invasive species surveillance. In this study, we develop a multi-locus non-destructive metabarcoding protocol that allows sensitive detection of agricultural pests, and subsequent confirmation using traditional diagnostic techniques. We validate this protocol for the detection of tomato potato psyllid (Bactericera cockerelli) and Russian wheat aphid (Diuraphis noxia) within mock communities and field survey tra

SUBMITTER: Batovska J 

PROVIDER: S-EPMC8041782 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature