Preference of Proteomonas sulcata anion channelrhodopsin for NO₃^- revealed using a pH electrode method.

ABSTRACT: Ion channel proteins are physiologically important molecules in living organisms. Their molecular functions have been investigated using electrophysiological methods, which enable quantitative, precise and advanced measurements and thus require complex instruments and experienced operators. For simpler and easier measurements, we measured the anion transport activity of light-gated anion channelrhodopsins (ACRs) using a pH electrode method, which has already been established for ion pump rhodopsins. Using that method, we successfully measured the anion transport activity and its dependence on the wavelength of light, i.e. its action spectra, and on the anion species, i.e. its selectivity or preference, of several ACRs expressed in yeast cells. In addition, we identified the strong anion tr

SUBMITTER: Kikuchi C 

PROVIDER: S-EPMC8041784 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature