Carbohydrate binding module-fused antibodies improve the performance of cellulose-based lateral flow immunoassays.

ABSTRACT: Since the pandemic outbreak of Covid-19 in December 2019, several lateral flow assay (LFA) devices were developed to enable the constant monitoring of regional and global infection processes. Additionally, innumerable lateral flow test devices are frequently used for determination of different clinical parameters, food safety, and environmental factors. Since common LFAs rely on non-biodegradable nitrocellulose membranes, we focused on their replacement by cellulose-composed, biodegradable papers. We report the development of cellulose paper-based lateral flow immunoassays using a carbohydrate-binding module-fused to detection antibodies. Studies regarding the protein binding capacity and potential protein wash-off effects on cellulose paper demonstrated a 2.7-fold protein binding capacity

SUBMITTER: Elter A 

PROVIDER: S-EPMC8042022 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature