On-site processing of single chromosomal DNA molecules using optically driven microtools on a microfluidic workbench.

ABSTRACT: We developed optically driven microtools for processing single biomolecules using a microfluidic workbench composed of a microfluidic platform that functions under an optical microscope. The optically driven microtools have enzymes immobilized on their surfaces, which catalyze chemical reactions for molecular processing in a confined space. Optical manipulation of the microtools enables them to be integrated with a microfluidic device for controlling the position, orientation, shape of the target sample. Here, we describe the immobilization of enzymes on the surface of microtools, the microfluidics workbench, including its microtool storage and sample positioning functions, and the use of this system for on-site cutting of single chromosomal DNA molecules. We fabricated microtools by UV li

SUBMITTER: Masuda A 

PROVIDER: S-EPMC8042024 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature