A microfluidic approach to rapid sperm recovery from heterogeneous cell suspensions.

ABSTRACT: The isolation of sperm cells from background cell populations and debris is an essential step in all assisted reproductive technologies. Conventional techniques for sperm recovery from testicular sperm extractions stagnate at the sample processing stage, where it can take several hours to identify viable sperm from a background of collateral cells such as white bloods cells (WBCs), red blood cells (RBCs), epithelial cells (ECs) and in some cases cancer cells. Manual identification of sperm from contaminating cells and debris is a tedious and time-consuming operation that can be suitably addressed through inertial microfluidics. Microfluidics has proven an effective technology for high-quality sperm selection based on motility. However, motility-based selection methods cannot cater for viab

SUBMITTER: Vasilescu SA 

PROVIDER: S-EPMC8042033 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature