Autonomous Purkinje cell activation instructs bidirectional motor learning through evoked dendritic calcium signaling.

ABSTRACT: The signals in cerebellar Purkinje cells sufficient to instruct motor learning have not been systematically determined. Therefore, we applied optogenetics in mice to autonomously excite Purkinje cells and measured the effect of this activity on plasticity induction and adaptive behavior. Ex vivo, excitation of channelrhodopsin-2-expressing Purkinje cells elicits dendritic Ca²⁺ transients with high-intensity stimuli initiating dendritic spiking that additionally contributes to the Ca²⁺ response. Channelrhodopsin-2-evoked Ca²⁺ transients potentiate co-active parallel fiber synapses; depression occurs when Ca²⁺ responses were enhanced by dendritic spiking. In vivo, optogenetic Purkinje cell activation drives an adaptive decrease in vestibulo-ocular

SUBMITTER: Bonnan A 

PROVIDER: S-EPMC8042043 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature