Whole genome deep sequencing analysis of viral quasispecies diversity and evolution in HBeAg seroconverters.

ABSTRACT:

Background & aims

We aimed to investigate how viral quasispecies of the HBV whole genome evolves and diversifies in response to HBeAg seroconversion and viral control utilising next-generation sequencing (NGS).

Methods

Fifty HBeAg-positive chronic hepatitis B patients, including 18 treatment-naïve and 32 interferon (IFN)-treated individuals, were recruited. Serial HBV whole genomes in serum were analysed by NGS to determine sequence characteristics and viral quasispecies.

Results

HBV quasispecies diversity, measured by nucleotide diversity, was negatively correlated with viral load and hepatitis activity. Spontaneous HBeAg seroconverters exhibited significantly greater viral quasispecies diversity than treatment-naïve non-seroconverters from >1 year before seroconvers

SUBMITTER: Lin SR 

PROVIDER: S-EPMC8042178 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature