High-throughput nuclear magnetic resonance measurement of citrate in serum and plasma in the clinical laboratory.

ABSTRACT:

Objectives

Despite reports highlighting citrate association with different diseases, serum citrate is scarcely used for diagnosis. Existing methods to quantify citrate are limited by their complexity and practicality of implementation. A simple and rapid NMR-based method to measure circulating citrate is described here, and its analytical performance evaluated.

Design

and Methods: Citrate was quantified from NMR spectra using a non-negative linear least squares deconvolution algorithm. The analytical characteristics of the assay were evaluated using CLSI guidelines. To determine if the assay has adequate sensitivity to measure clinically relevant concentrations of citrate, the assay was used to quantify citrate in apparently healthy adults (n ​= ​553), and in the general pop

SUBMITTER: Garcia E 

PROVIDER: S-EPMC8042410 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature