Screening for alternative splicing of lncRNA Dleu2 in the mouse liver cell line AML-12.

ABSTRACT: The long non coding RNA deleted in leukemia 2 gene (Dleu2) has recently been demonstrated to be an active player in the progression of several types of cancer, including hepatocellular carcinoma. Dleu2 may serve a role in modulating the downstream effects-mediated by alternative splicing of its multiple exons. However, the proportional expression of these alternative splicing populations of the Dleu2 exons is currently unknown. To determine how Dleu2 could be affected by alternative splicing, a series of alternative splicing primer sets were designed to investigate which transcripts were preferentially activated when Dleu2 was targeted for downregulation or upregulation. A specific Dleu2 small interfering RNA that targeted an exon upstream of the tumor suppressor microRNA site significantl

SUBMITTER: Kay MK 

PROVIDER: S-EPMC8042668 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature