Farnesoid X receptor via Notch1 directs asymmetric cell division of Sox9⁺ cells to prevent the development of liver cancer in a mouse model.

ABSTRACT:

Background

Asymmetrical cell division (ACD) maintains the proper number of stem cells to ensure self-renewal. The rate of symmetric division increases as more cancer stem cells (CSCs) become malignant; however, the signaling pathway network involved in CSC division remains elusive. FXR (Farnesoid X receptor), a ligand-activated transcription factor, has several anti-tumor effects and has been shown to target CSCs. Here, we aimed at evaluating the role of FXR in the regulation of the cell division of CSCs.

Methods

The FXR target gene and downstream molecular mechanisms were confirmed by qRT-PCR, Western blot, luciferase reporter assay, EMAS, Chip, and IF analyses. Pulse-chase BrdU labeling and paired-cell experiments were used to detect the cell division of liver CSCs. Gain-

SUBMITTER: Chen M 

PROVIDER: S-EPMC8042944 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature