Ligand Binding Prolongs Androgen Receptor Protein Half-Life by Reducing its Degradation.

ABSTRACT: Androgens are important in female reproduction, but the molecular actions of androgens in female reproductive tissues are not fully understood. We investigated the androgen-responsive transcriptome in human and mouse granulosa cells (GCs) and surprisingly found that the gene-regulation activity of androgen receptor (AR) in these cells is negligible. We then investigated extranuclear actions of AR and found that in human and mouse GCs, as well as in prostate cancer cells, dihydrotestosterone (DHT) dramatically increases the half-life of its own receptor protein. Using the human granulosa-like KGN cells, we show that this effect is not the result of increased AR gene transcription or protein synthesis, nor is it fully abrogated by proteasome inhibition. Knockdown of PTEN, which contri

SUBMITTER: Astapova O 

PROVIDER: S-EPMC8043068 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature