Cardiovascular disease risk factors induce mesenchymal features and senescence in mouse cardiac endothelial cells.

ABSTRACT: Aging, obesity, hypertension, and physical inactivity are major risk factors for endothelial dysfunction and cardiovascular disease (CVD). We applied fluorescence-activated cell sorting (FACS), RNA sequencing, and bioinformatic methods to investigate the common effects of CVD risk factors in mouse cardiac endothelial cells (ECs). Aging, obesity, and pressure overload all upregulated pathways related to TGF-β signaling and mesenchymal gene expression, inflammation, vascular permeability, oxidative stress, collagen synthesis, and cellular senescence, whereas exercise training attenuated most of the same pathways. We identified collagen chaperone Serpinh1 (also called as Hsp47) to be significantly increased by aging and obesity and repressed by exercise training. Mechanistic stu

SUBMITTER: Hemanthakumar KA 

PROVIDER: S-EPMC8043751 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature