Single cell transcriptional and chromatin accessibility profiling redefine cellular heterogeneity in the adult human kidney.

ABSTRACT: The integration of single cell transcriptome and chromatin accessibility datasets enables a deeper understanding of cell heterogeneity. We performed single nucleus ATAC (snATAC-seq) and RNA (snRNA-seq) sequencing to generate paired, cell-type-specific chromatin accessibility and transcriptional profiles of the adult human kidney. We demonstrate that snATAC-seq is comparable to snRNA-seq in the assignment of cell identity and can further refine our understanding of functional heterogeneity in the nephron. The majority of differentially accessible chromatin regions are localized to promoters and a significant proportion are closely associated with differentially expressed genes. Cell-type-specific enrichment of transcription factor binding motifs implicates the activation of NF-κB that promo

SUBMITTER: Muto Y 

PROVIDER: S-EPMC8044133 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature