Tandem UGT71B5s Catalyze Lignan Glycosylation in Isatis indigotica With Substrates Promiscuity.

ABSTRACT: Lignans are a class of chemicals formed by the combination of two molecules of phenylpropanoids with promising nutritional and pharmacological activities. Lignans glucosides, which are converted from aglycones catalyzed by uridine diphosphate (UDP) glycosyltransferases (UGTs), have abundant bioactivities. In the present study, two UGTs from Isatis indigotica Fort., namely IiUGT71B5a and IiUGT71B5b, were characterized to catalyze the glycosylation of lignans with promiscuities toward various sugar acceptors and sugar donors, and pinoresinol was the preferred substrate. IiUGT71B5a was capable of efficiently producing both pinoresinol monoglycoside and diglycoside. However, IiUGT71B5b only produced monoglycoside, and exhibited considerably lower activity tha

SUBMITTER: Chen X 

PROVIDER: S-EPMC8044456 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature