Detection and quantification of EGFR T790M mutation in liquid biopsies by droplet digital PCR.

ABSTRACT:

Background

Liquid biopsy allows the identification of targetable cancer mutations in a minimally invasive manner. In patients with advanced non-small cell lung cancer (NSCLC), droplet digital PCR (ddPCR) is increasingly used to genotype the epidermal growth factor receptor (EGFR) gene in circulating cell-free DNA (cfDNA). However, the sensitivity of this method is still under debate. The aim of this study was to implement and assess the performance of a ddPCR assay for detecting the EGFR T790M mutation in liquid biopsies.

Methods

A ddPCR assay was optimized to detect the EGFR T790M mutation in plasma samples from 77 patients with NSCLC in progression.

Results

Our ddPCR assay enabled the detection and quantification of the EGFR T790M mutatio

SUBMITTER: Silveira C 

PROVIDER: S-EPMC8044487 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature