Mapping Solute Clearance From the Mouse Hippocampus Using a 3D Imaging Cryomicrotome.

ABSTRACT: The hippocampus is susceptible to protein aggregation in neurodegenerative diseases such as Alzheimer's disease. This protein accumulation is partially attributed to an impaired clearance; however, the removal pathways for fluids and waste products are not fully understood. The aim of this study was therefore to map the clearance pathways from the mouse brain. A mixture of two fluorescently labeled tracers with different molecular weights was infused into the hippocampus. A small subset of mice (n = 3) was sacrificed directly after an infusion period of 10 min to determine dispersion of the tracer due to the infusion, while another group was sacrificed after spreading of the tracers for an additional 80 min (n = 7). Upon sacrifice, mice were frozen and sectioned as a whole by

SUBMITTER: Naessens DMP 

PROVIDER: S-EPMC8044999 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature