Automated and customizable quantitative image analysis of whole Caenorhabditis elegans germlines.

ABSTRACT: Arranged in a spatial-temporal gradient for germ cell development, the adult germline of Caenorhabditis elegans is an excellent system for understanding the generation, differentiation, function, and maintenance of germ cells. Imaging whole C. elegans germlines along the distal-proximal axis enables powerful cytological analyses of germ cell nuclei as they progress from the pre-meiotic tip through all the stages of meiotic prophase I. To enable high-content image analysis of whole C. elegans gonads, we developed a custom algorithm and pipelines to function with image processing software that enables: (1) quantification of cytological features at single nucleus resolution from immunofluorescence images; and (2) assessment of these individual nuclei based on their position within the germlin

SUBMITTER: Toraason E 

PROVIDER: S-EPMC8045727 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature