Global coordination of metabolic pathways in Escherichia coli by active and passive regulation.

ABSTRACT: Microorganisms adjust metabolic activity to cope with diverse environments. While many studies have provided insights into how individual pathways are regulated, the mechanisms that give rise to coordinated metabolic responses are poorly understood. Here, we identify the regulatory mechanisms that coordinate catabolism and anabolism in Escherichia coli. Integrating protein, metabolite, and flux changes in genetically implemented catabolic or anabolic limitations, we show that combined global and local mechanisms coordinate the response to metabolic limitations. To allocate proteomic resources between catabolism and anabolism, E. coli uses a simple global gene regulatory program. Surprisingly, this program is largely implemented by a single transcription factor, Crp, which directly activate

SUBMITTER: Kochanowski K 

PROVIDER: S-EPMC8045939 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature