Platypus: an open-access software for integrating lymphocyte single-cell immune repertoires with transcriptomes.

ABSTRACT: High-throughput single-cell sequencing (scSeq) technologies are revolutionizing the ability to molecularly profile B and T lymphocytes by offering the opportunity to simultaneously obtain information on adaptive immune receptor repertoires (VDJ repertoires) and transcriptomes. An integrated quantification of immune repertoire parameters, such as germline gene usage, clonal expansion, somatic hypermutation and transcriptional states opens up new possibilities for the high-resolution analysis of lymphocytes and the inference of antigen-specificity. While multiple tools now exist to investigate gene expression profiles from scSeq of transcriptomes, there is a lack of software dedicated to single-cell immune repertoires. Here, we present Platypus, an open-source software platform providing a u

SUBMITTER: Yermanos A 

PROVIDER: S-EPMC8046018 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature