The Inter-Relation between Leptin Receptor (Q223R) Gene Polymorphism and the Risk of Egyptian Patients with HCC.

ABSTRACT:

Background

The relationship of leptin (LEP) and polymorphism of leptin receptor (LEPR) were studied in patients with hepatocellular carcinoma (HCC) and compared with those with liver cirrhosis to find out the extent of the risk of LEPR on patients with HCC.

Methods

Serum LEP level and LEPR Q223R gene polymorphism were determined in 300 patients with liver disease categorized equally into five groups' healthy volunteers, patients with hepatitis C (HCV), patients with non-alcoholic steatohepatitis (NASH),  liver cirrhosis and HCC. LEPR gene was amplified by polymerase chain reaction (PCR) then digested by the MSP1 restriction enzyme.

Results

The isolated 212 bp of LEPR was sequenced. The serum LEP level was reduced in patients with cirrhotic and HCC. Serum LEP level had

SUBMITTER: Karam HA 

PROVIDER: S-EPMC8046304 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature