Identification of Inflammatory Genes, Pathways, and Immune Cells in Necrotizing Enterocolitis of Preterm Infant by Bioinformatics Approaches.

ABSTRACT:

Background

Necrotizing enterocolitis (NEC) is one of the most serious gastrointestinal disease-causing high morbidity and mortality in premature infants. However, the underlying mechanism of the pathogenesis of NEC is still not fully understood.

Methods

RNA sequencing of intestinal specimens from 9 NEC and 5 controls was employed to quantify the gene expression levels. RNA sequencing was employed to quantify the gene expression levels. DESeq2 tool was used to identify the differentially expressed genes. The biological function, pathways, transcription factors, and immune cells dysregulated in NEC were characterized by gene set enrichment analysis.

Results

In the present study, we analyzed RNA sequencing data of NECs and controls and revealed that immune-related pathwa

SUBMITTER: Zhang L 

PROVIDER: S-EPMC8046524 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature