Complement Initiation Varies by Sex in Intestinal Ischemia Reperfusion Injury.

ABSTRACT: Intestinal ischemia reperfusion (IR)-induced tissue injury represents an acute inflammatory response with significant morbidity and mortality. The mechanism of IR-induced injury is not fully elucidated, but recent studies suggest a critical role for complement activation and for differences between sexes. To test the hypothesis that complement initiation differs by sex in intestinal IR, we performed intestinal IR on male and female WT C57B6L/, C1q^-/-, MBL^-/-, or properdin (P)^-/- mice. Intestinal injury, C3b and C5a production and ex vivo secretions were analyzed. Initial studies demonstrated a difference in complement mRNA and protein in male and female WT mice. In response to IR, male C1q-, MBL- and P-deficient mice sustained less injury than male W

SUBMITTER: Wu M 

PROVIDER: S-EPMC8047102 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature