Unbiased proteomic profiling of host cell extracellular vesicle composition and dynamics upon HIV-1 infection.

ABSTRACT: Cells release diverse types of extracellular vesicles (EVs), which transfer complex signals to surrounding cells. Specific markers to distinguish different EVs (e.g. exosomes, ectosomes, enveloped viruses like HIV) are still lacking. We have developed a proteomic profiling approach for characterizing EV subtype composition and applied it to human Jurkat T cells. We generated an interactive database to define groups of proteins with similar profiles, suggesting release in similar EVs. Biochemical validation confirmed the presence of preferred partners of commonly used exosome markers in EVs: CD81/ADAM10/ITGB1, and CD63/syntenin. We then compared EVs from control and HIV-1-infected cells. HIV infection altered EV profiles of several cellular proteins, including MOV10 and SPN, which became in

SUBMITTER: Martin-Jaular L 

PROVIDER: S-EPMC8047442 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature