Contextualizing the biological relevance of standardized high-resolution respirometry to assess mitochondrial function in permeabilized human skeletal muscle.

ABSTRACT:

Aim

This study sought to provide a statistically robust reference for measures of mitochondrial function from standardized high-resolution respirometry with permeabilized human skeletal muscle (ex vivo), compare analogous values obtained via indirect calorimetry, arterial-venous O₂ differences and ³¹ P magnetic resonance spectroscopy (in vivo) and attempt to resolve differences across complementary methodologies as necessary.

Methods

Data derived from 831 study participants across research published throughout March 2009 to November 2019 were amassed to examine the biological relevance of ex vivo assessments under standard conditions, ie physiological temperatures of 37°C and respiratory chamber oxygen concentrations of ~250 to 500 μmol/L.

Results

SUBMITTER: Jacobs RA 

PROVIDER: S-EPMC8047922 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature