Identification of DNA methylation markers for early detection of CRC indicates a role for nervous system-related genes in CRC.

ABSTRACT:

Purpose

Colonoscopy and the fecal immunochemical test (FIT) are currently the most widely used screening modalities for colorectal cancer (CRC), however, both with their own limitations. Here we aim to identify and validate stool-based DNA methylation markers for the early detection of CRC and investigate the biological pathways prone to DNA methylation.

Methods

DNA methylation marker discovery was performed using The Cancer Genome Atlas (TCGA) colon adenocarcinoma data set consisting of normal and primary colon adenocarcinoma tissue. The performance of the five best candidate markers and a previously identified marker, NDRG4, was evaluated on tissues and whole stool samples of healthy subjects and CRC patients using quantitative MSP assays. The results were compared and com

SUBMITTER: Rademakers G 

PROVIDER: S-EPMC8048074 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature