Mesophasic organization of GABA_A receptors in hippocampal inhibitory synapses.

ABSTRACT: Information processing in the brain depends on specialized organization of neurotransmitter receptors and scaffolding proteins within the postsynaptic density. However, how these molecules are organized in situ remains largely unknown. In this study, template-free classification of oversampled sub-tomograms was used to analyze cryo-electron tomograms of hippocampal synapses. We identified type-A GABA receptors (GABA_ARs) in inhibitory synapses and determined their in situ structure at 19-Å resolution. These receptors are organized hierarchically: from GABA_AR super-complexes with a preferred inter-receptor distance of 11 nm but variable relative angles, through semi-ordered, two-dimensional receptor networks with reduced Voronoi entropy, to mesophasic assembly with a sh

SUBMITTER: Liu YT 

PROVIDER: S-EPMC8048127 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature