Phylogenetic analysis of Harmonin homology domains.

ABSTRACT:

Background

Harmonin Homogy Domains (HHD) are recently identified orphan domains of about 70 residues folded in a compact five alpha-helix bundle that proved to be versatile in terms of function, allowing for direct binding to a partner as well as regulating the affinity and specificity of adjacent domains for their own targets. Adding their small size and rather simple fold, HHDs appear as convenient modules to regulate protein-protein interactions in various biological contexts. Surprisingly, only nine HHDs have been detected in six proteins, mainly expressed in sensory neurons.

Results

Here, we built a profile Hidden Markov Model to screen the entire UniProtKB for new HHD-containing proteins. Every hit was manually annotated, using a clustering approach, confirming that on

SUBMITTER: Colcombet-Cazenave B 

PROVIDER: S-EPMC8048344 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature