Creation and filtering of a recurrent spectral library of CHO cell metabolites and media components.

ABSTRACT: This paper reports the first implementation of a new type of mass spectral library for the analysis of Chinese hamster ovary (CHO) cell metabolites that allows users to quickly identify most compounds in any complex metabolite sample. We also describe an annotation methodology developed to filter out artifacts and low-quality spectra from recurrent unidentified spectra of metabolites. CHO cells are commonly used to produce biological therapeutics. Metabolic profiles of CHO cells and media can be used to monitor process variability and look for markers that discriminate between batches of product. We have created a comprehensive library of both identified and unidentified metabolites derived from CHO cells that can be used in conjunction with tandem mass spectrometry to identify metabolites

SUBMITTER: Telu KH 

PROVIDER: S-EPMC8048470 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature