Sensitive quantification of dipicolinic acid from bacterial endospores in soils and sediments.

ABSTRACT: Endospore-forming bacteria make up an important and numerically significant component of microbial communities in a range of settings including soils, industry, hospitals and marine sediments extending into the deep subsurface. Bacterial endospores are non-reproductive structures that protect DNA and improve cell survival during periods unfavourable for bacterial growth. An important determinant of endospores withstanding extreme environmental conditions is 2,6-pyridine dicarboxylic acid (i.e. dipicolinic acid, or DPA), which contributes heat resistance. This study presents an improved HPLC-fluorescence method for DPA quantification using a single 10-min run with pre-column Tb³⁺ chelation. Relative to existing DPA quantification methods, specific improvements pertain to sensitiv

SUBMITTER: Rattray JE 

PROVIDER: S-EPMC8048543 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature