Uncoupling growth and succinic acid production in an industrial Saccharomyces cerevisiae strain.

ABSTRACT: This study explores the relation between biomass-specific succinic acid (SA) production rate and specific growth rate of an engineered industrial strain of Saccharomyces cerevisiae, with the aim to investigate the extent to which growth and product formation can be uncoupled. Ammonium-limited aerobic chemostat and retentostat cultures were grown at different specific growth rates under industrially relevant conditions, that is, at a culture pH of 3 and with sparging of a 1:1 CO₂ -air mixture. Biomass-specific SA production rates decreased asymptotically with decreasing growth rate. At near-zero growth rates, the engineered strain maintained a stable biomass-specific SA production rate for over 500 h, with a SA yield on glucose of 0.61 mol mol^-1 . These results demonst

SUBMITTER: Liu Y 

PROVIDER: S-EPMC8048565 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature