Ontology highlight
ABSTRACT:
SUBMITTER: Liebscher S
PROVIDER: S-EPMC8048679 | biostudies-literature | 2021 Apr
REPOSITORIES: biostudies-literature
Liebscher Sandra S Mathea Sebastian S Aumüller Tobias T Pech Andreas A Bordusa Frank F
Chembiochem : a European journal of chemical biology 20210115 7
Fluorescent fusion proteins are powerful tools for studying biological processes in living cells, but universal application is limited due to the voluminous size of those tags, which might have an impact on the folding, localization or even the biological function of the target protein. The designed biocatalyst trypsiligase enables site-directed linkage of small-sized fluorescence dyes on the N terminus of integral target proteins located in the outer membrane of living cells through a stable na ...[more]