Optimised tissue clearing minimises distortion and destruction during tissue delipidation.

ABSTRACT:

Aims

A variety of tissue clearing techniques have been developed to render intact tissue transparent. For thicker samples, additional partial tissue delipidation is required before immersion into the final refractive index (RI)-matching solution, which alone is often inadequate to achieve full tissue transparency. However, it is difficult to determine a sufficient degree of tissue delipidation, excess of which can result in tissue distortion and protein loss. Here, we aim to develop a clearing strategy that allows better monitoring and more precise determination of delipidation progress.

Methods

We combined the detergent sodium dodecyl sulphate (SDS) with OPTIClear, a RI-matching solution, to form a strategy termed Accurate delipidation with Optimal Clearing (Accu-OptiCleari

SUBMITTER: Lee K 

PROVIDER: S-EPMC8048831 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature