Ingestion of Bifidobacterium longum changes miRNA levels in the brains of mice.

ABSTRACT: The purpose of this research is to investigate the relationship between the microbiota of the gastrointestinal (GI) system and relative gene expression of miRNAs and mRNAs in the brain. C57BL/6 mice and Balb/c mice are fed Bifidobacterium longum, a well-characterized probiotic bacterial species shown to change behavior and improve sociability of Balb/c mice. After feeding, RNA was extracted from whole brains and PCR arrays were utilized to determine changes in the gene expression of brain-specific miRNAs. The results of these PCR arrays reveal that the relative gene expression of mmu-mir-652-3p is sensitive to B. longum probiotic treatment in C57BL/6 mice. qPCR was performed to measure expression of Dab1, an mRNA target of this miRNA. Dab1 expression is also dependent on B. longum. The goa

SUBMITTER: DeVries L 

PROVIDER: S-EPMC8049326 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature