Quantification of Macrophage-Driven Inflammation During Myocardial Infarction with ¹⁸F-LW223, a Novel TSPO Radiotracer with Binding Independent of the rs6971 Human Polymorphism.

ABSTRACT: Myocardial infarction (MI) is one of the leading causes of death worldwide, and inflammation is central to tissue response and patient outcomes. The 18-kDa translocator protein (TSPO) has been used in PET as an inflammatory biomarker. The aims of this study were to screen novel, fluorinated, TSPO radiotracers for susceptibility to the rs6971 genetic polymorphism using in vitro competition binding assays in human brain and heart; assess whether the in vivo characteristics of our lead radiotracer, ¹⁸F-LW223, are suitable for clinical translation; and validate whether ¹⁸F-LW223 can detect macrophage-driven inflammation in a rat MI model. Methods: Fifty-one human brain and 29 human heart tissue samples were screened for the rs6971 polymorphism. Competition binding

SUBMITTER: MacAskill MG 

PROVIDER: S-EPMC8049364 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature