The effects of extracellular matrix rigidity on 3-dimensional cultures of amnion membrane cells.

ABSTRACT:

Introduction

To determine 3D growth of amnion membrane cells using soft substrate plates of various rigidities.

Methods

Amnion epithelial (AEC) and mesenchymal cells (AMC) were cultured on 6-well soft substrate plates coated with matrigel and elastomer with rigidities of 0.5, 2, 8, 16, and 64 kPa (n = 3 each). Controls were cells in standard culture conditions. Cell morphology, spheroids' and sheets' formations and viability (bright field microscopy and crystal violet staining), and cellular transitions (vimentin/cytokeratin-18 [CK-18] ratios) were analyzed. A Student t-test was used for statistical analyses.

Results

AECs in substrate rigidities between 2 and 8 kPa formed 3D features (spheroids and sheets) while retaining viability. Two kPa produced spheroids with epi

SUBMITTER: Richardson LS 

PROVIDER: S-EPMC8050838 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature