IκBα targeting promotes oxidative stress-dependent cell death.

ABSTRACT:

Background

Oxidative stress is a hallmark of many cancers. The increment in reactive oxygen species (ROS), resulting from an increased mitochondrial respiration, is the major cause of oxidative stress. Cell fate is known to be intricately linked to the amount of ROS produced. The direct generation of ROS is also one of the mechanisms exploited by common anticancer therapies, such as chemotherapy.

Methods

We assessed the role of NFKBIA with various approaches, including in silico analyses, RNA-silencing and xenotransplantation. Western blot analyses, immunohistochemistry and RT-qPCR were used to detect the expression of specific proteins and genes. Immunoprecipitation and pull-down experiments were used to evaluate protein-protein interactions.

Results

Here, by using a

SUBMITTER: Carra G 

PROVIDER: S-EPMC8050912 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature