Engineered sex ratio distortion by X-shredding in the global agricultural pest Ceratitis capitata.

ABSTRACT:

Background

Genetic sex ratio distorters are systems aimed at effecting a bias in the reproductive sex ratio of a population and could be applied for the area-wide control of sexually reproducing insects that vector disease or disrupt agricultural production. One example of such a system leading to male bias is X-shredding, an approach that interferes with the transmission of the X-chromosome by inducing multiple DNA double-strand breaks during male meiosis. Endonucleases targeting the X-chromosome and whose activity is restricted to male gametogenesis have recently been pioneered as a means to engineer such traits.

Results

Here, we enabled endogenous CRISPR/Cas9 and CRISPR/Cas12a activity during spermatogenesis of the Mediterranean fruit fly Ceratitis capitata, a worldwide a

SUBMITTER: Meccariello A 

PROVIDER: S-EPMC8051031 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature