Transcriptional profiling of fission yeast RNA polymerase II CTD mutants.

ABSTRACT: The carboxyl-terminal domain (CTD) of RNA polymerase II consists of tandem repeats of heptapeptide Y¹S²P³T⁴S⁵P⁶S⁷ The CTD recruits proteins that drive or regulate gene expression. The trafficking of CTD-interacting proteins is orchestrated by remodeling CTD primary structure via Ser/Thr/Tyr phosphorylation and proline cis-trans isomerization, which collectively inscribe a CTD code. The fission yeast CTD consists of 29 repeats. To decipher the output of the fission yeast CTD code, we manipulated CTD length and amino acid content and gauged effects of these changes on gene expression. Whereas deleting 11 heptads has no effect on yeast growth, RNA-seq revealed that 25% of protein-coding transcripts were dysreg

SUBMITTER: Garg A 

PROVIDER: S-EPMC8051263 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature