Structure of 3'-PO₄/5'-OH RNA ligase RtcB in complex with a 5'-OH oligonucleotide.

ABSTRACT: RtcB enzymes comprise a widely distributed family of manganese- and GTP-dependent RNA repair enzymes that join 2',3'-cyclic phosphate ends to 5'-OH ends via RtcB-(histidinyl-N)-GMP, RNA 3'-phosphate, and RNA_3'pp_5'G intermediates. RtcB can ligate either 5'-OH RNA or 5'-OH DNA strands in vitro. The nucleic acid contacts of RtcB are uncharted. Here we report a 2.7 Å crystal structure of Pyrococcus horikoshii RtcB in complex with a 6-mer 5'-OH DNA oligonucleotide _HOA¹pT²pG³pT⁴pC⁵pC⁶, which reveals enzymic contacts of Asn202 to the terminal 5'-OH nucleophile; Arg238 to the A¹pT² and T²pG³ phosphates; Arg190 and Gln194 to the T²pG^3<

SUBMITTER: Banerjee A 

PROVIDER: S-EPMC8051266 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature