SUV39H2 controls trophoblast stem cell fate.

ABSTRACT:

Background

The placenta is formed by the coordinated expansion and differentiation of trophoblast stem (TS) cells along a multi-lineage pathway. Dynamic regulation of histone 3 lysine 9 (H3K9) methylation is pivotal to cell differentiation for many cell lineages, but little is known about its involvement in trophoblast cell development.

Methods

Expression of H3K9 methyltransferases was surveyed in rat TS cells maintained in the stem state and following differentiation. The role of suppressor of variegation 3-9 homolog 2 (SUV39H2) in the regulation of trophoblast cell lineage development was investigated using a loss-of-function approach in rat TS cells and ex vivo cultured rat blastocysts.

Results

Among the twelve-known H3K9 methyltransferases, only SUV39H2 exhibited

SUBMITTER: Wang L 

PROVIDER: S-EPMC8052280 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature