Global deletion of NTPDase3 protects against diet-induced obesity by increasing basal energy metabolism.

ABSTRACT:

Background

Ecto-nucleoside triphosphate diphosphohydrolase 3 (NTPDase3), also known as CD39L3, is the dominant ectonucleotidase expressed by beta cells in the islet of Langerhans and on nerves. NTPDase3 catalyzes the conversion of extracellular ATP and ADP to AMP and modulates purinergic signaling. Previous studies have shown that NTPDase3 decreases insulin release from beta-cells in vitro. This study aims to determine the impact of NTPDase3 in diet-induced obesity (DIO) and metabolism in vivo.

Methods

We developed global NTPDase3 deficient (Entpd3^-/-) and islet beta-cell-specific NTPDase-3 deficient mice (Entpd3^{flox/flox,InsCre}) using Ins1-Cre targeted gene editing to compare metabolic phenotypes with wildtype (WT) mice on a high-fat diet (HFD).

Res

SUBMITTER: Sandhu B 

PROVIDER: S-EPMC8052311 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature