Experimental parameters defining ultra-low biomass bioaerosol analysis.

ABSTRACT: Investigation of the microbial ecology of terrestrial, aquatic and atmospheric ecosystems requires specific sampling and analytical technologies, owing to vastly different biomass densities typically encountered. In particular, the ultra-low biomass nature of air presents an inherent analytical challenge that is confounded by temporal fluctuations in community structure. Our ultra-low biomass pipeline advances the field of bioaerosol research by significantly reducing sampling times from days/weeks/months to minutes/hours, while maintaining the ability to perform species-level identification through direct metagenomic sequencing. The study further addresses all experimental factors contributing to analysis outcome, such as amassment, storage and extraction, as well as factors that impact o

SUBMITTER: Luhung I 

PROVIDER: S-EPMC8052325 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature